A Trichinella spiralis new born larvae-specific protein, Ts-NBL1, interacts with host’s cell vimentin

作者：A. Wang, Xiaohao Liu, Aurélie Heckmann, Grégory Caignard, Damien Vitour, Edouard Hirchaud, M LIU, Pascal Boireau, Grégory Karadjian, Isabelle Vallée · 发表于：Parasitology Research · 年份：2022 · DOI：10.1007/s00436-022-07479-7 · 被引用次数：6 · 研究领域：Parasitic Diseases Research and Treatment、Insect symbiosis and bacterial influences、Parasites and Host Interactions

The parasitic nematode Trichinella has a special relationship with its host as it has a unique intracellular location within the feeder cell which is a structure derived from skeletal muscle fiber. It has been proposed that "parakines" secreted by Trichinella larvae serve as messengers to implement communication between the parasite and the muscle cells through a molecular cross-talk to ensure permanent coexistence within the host. The Ts-NBL1 protein is considered to be a potential key "parakine" involved in the early invasion of the muscle fiber and its transformation into a feeder cell during Trichinella spiralis infection. This study used for the first time yeast two-hybrid (Y2H) technology in Trichinella to identify Ts-NBL1 interacting proteins. GST co-affinity purification experiments confirmed vimentin as an important interactor. The discovery of the new host proteins interacting with Ts-NBL1 will help to suggest that Ts-NBL1 contributes to participate in the capsule formation of feeder cells and provide ideas for understanding the molecular and cellular mechanisms involved in the survival of Trichinella in the host.