Endogenous IL-10 Contributes to Wound Healing and Regulates Tissue Repair

作者：Walker D. Short, Meredith Rae, Thomas Chengxuan Lu, Benjamin Padon, Tanuj J. Prajapati, Fayiz Faruk, Oluyinka O. Olutoye, Ling Yu, Paul L. Bollyky, Sundeep G. Keswani, Swathi Balaji · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2022 · DOI：10.1101/2022.03.15.484452 · 被引用次数：2 · 研究领域：Wound Healing and Treatments、Pressure Ulcer Prevention and Management、Tendon Structure and Treatment

Abstract Background Interleukin-10 (IL-10) is essential in fetal regenerative wound healing and likewise promotes a regenerative phenotype in adult dermal wounds. However, the role of endogenous IL-10 in postnatal dermal wound healing is not well established. We sought to determine the role of IL-10 in murine full thickness, excisional wounds that are splinted to prevent contracture and mimic human patterns of wound closure. Methods Full thickness, excisional wounds were made in wildtype (WT) and IL-10 -/- mice on a C57BL/6J background (F/M, 8wks old). In a subset of wounds, contraction was prevented by splinting with silicone stents (stenting) and maintaining a moist wound microenvironment using a semi-occlusive dressing. Wounds were examined for re-epithelialization, granulation tissue deposition, and inflammatory cell infiltrate at day 7 and fibrosis and scarring at day 30 post-wounding. Results We observed no difference in wound healing rate between WT and IL-10 -/- mice in either the stented or unstented group. At day 7, unstented IL-10 -/- wounds had a larger granulation tissue area and more inflammatory infiltrate than their WT counterparts. However, we did observe more F4/80 + cell infiltrate in stented IL-10 -/- wounds at day 7. At day 30, stented wounds had increased scar area and epithelial thickness compared to unstented wounds. Conclusions These data suggest that endogenous IL-10 expression does not alter closure of full thickness excisional wounds when wound hyd...