Dietary chromium could improve growth, antioxidant capacity, chromium accumulation in tissues and expression of genes involved into glucose and lipid metabolism in juvenile mud crab Scylla paramamosain

作者：Yingying Zhang, Jiaxiang Luo, Tingting Zhu, Xiangsheng Zhang, Min Jin, Lefei Jiao, Fanyi Meng, Cláudia Figueiredo-Silva, Yucong Hong, Qicun Zhou · 发表于：Aquaculture Reports · 年份：2022 · DOI：10.1016/j.aqrep.2022.101088 · 被引用次数：26 · 研究领域：Environmental Toxicology and Ecotoxicology、Chromium effects and bioremediation、Iron Metabolism and Disorders

Chromium (Cr) is an essential micronutrient for all animals, which is also an important component of glucose tolerance factor. The present study was conducted to evaluate the effect of dietary Cr levels on growth, Cr accumulation in tissues, glucose and lipid metabolism and insulin signal pathway of juvenile mud crab. Six isonitrogenous and isolipidic diets were formulated to contain 1.4 (basal diet), 1.7, 2.0, 2.2, 2.6 and 3.7 mg kg−1 Cr, respectively. The results indicated that crabs fed with 2.2 and 2.6 mg kg−1 Cr diets had the highest percent weight gain (PWG) and specific growth rate (SGR) among all treatments. Cr concentration in hepatopancreas significantly increased with increase of dietary Cr levels. Crabs fed with 3.7 mg kg−1 Cr diet exhibited lower antioxidant enzyme activities and higher malondialdehyde (MDA) in hepatopancreas and hemolymph than those fed the other diets. Crabs fed the diets with 2.2 and 2.6 mg kg−1 Cr showed higher activities of hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK). The expression levels of genes related to insulin signaling pathway, glycolysis and fat synthesis, glycolipid metabolism (such as phosphatidylinositol 3-kinase (pi3k), hexokinase (hk), and fatty acid synthase (fas)) in hepatopancreas were significantly up-regulated. The expression levels of genes related to gluconeogenesis and lipolysis such as fructose-1,6-bisphosphatase (fbp) and carnitine palmitoyltransferase 1 (cpt1) were significantly down-regulated...