Development and Validation of a Competitive ELISA Based on Bovine Monoclonal Antibodies for the Detection of Neutralizing Antibodies against Foot-and-Mouth Disease Virus Serotype A

作者：Yimei Cao, Kun Li, Xiangchuan Xing, Guoqiang Zhu, Yuanfang Fu, Huifang Bao, Xingwen Bai, Pu Sun, Pinghua Li, Jing Zhang, Xueqing Ma, Jian Wang, Zhixun Zhao, Dong Li, Zaixin Liu, Zengjun Lu · 发表于：Journal of Clinical Microbiology · 年份：2022 · DOI：10.1128/jcm.02142-21 · 被引用次数：25 · 研究领域：Animal Disease Management and Epidemiology、Viral Infections and Immunology Research、Vector-Borne Animal Diseases

The level of neutralizing antibodies in vaccinated animals is directly related to their level of protection against a virus challenge. The virus neutralization test (VNT) is a “gold standard” method for detecting neutralizing antibodies against foot-and-mouth disease virus (FMDV). However, VNT requires high-containment facilities that can handle live viruses and is not suitable for large-scale serological surveillance. In this study, a bovine broadly neutralizing monoclonal antibody (W145) against FMDV serotype A was successfully produced using fluorescence-based single-B-cell antibody technology.