Vitamin D promotes growth, feed utilization and gene expression related to lipid metabolism for juvenile Orange-spotted grouper Epinephelus coioides

作者：Huaxing Lin, Mingyan Ding, Gyan Watson Ray, Beiping Tan, Qihui Yang · 发表于：Aquaculture Reports · 年份：2022 · DOI：10.1016/j.aqrep.2022.101074 · 被引用次数：21 · 研究领域：Aquaculture Nutrition and Growth、Vitamin D Research Studies、Antioxidant Activity and Oxidative Stress

This study aimed to evaluate vitamin D (VD) levels on growth, feed utilization, enzymatic activity in lipid metabolism and mRNA expression for juvenile Orange-spotted grouper Epinephelus coioides (initial weight:7.40 ± 0.03 g) for 8 weeks. Six group fishes were fed diets containing different VD levels (0, 500, 1000, 2000, 4000, and 10,000 IU/kg, respectively). Compared to the control group, the weight gain rate and feed utilization rate showed a significant increase and then decreased ( P < 0.05). The highest activities of alkaline phosphatase and superoxide dismutase were observed in the groups supplemented with 4000 and 1000 IU/kg VD, respectively ( P < 0.05). With VD increased, triglyceride content gradually decreased ( P < 0.05), whereas high-density lipoprotein content initially increased and then decreased. By comparison, cholesterol and low-density lipoprotein contents were not affected by VD supplementation levels ( P > 0.05). VD supplementation levels significantly reduced liver fatty acid synthase activity ( P < 0.05), but significantly promoted lipoprotein lipase and hepatic lipase activities ( P < 0.05). As VD increased, liver VD deposition increased significantly in the 2000 IU/kg group. Fatty acid synthase ( fas ) mRNA expression was significantly down-regulated and then up-regulated ( P < 0.05), whereas hepatic lipase ( hl ) mRNA expression levels exhibited the opposite trend. In summary, based on the broken-line regression analysis of WGR and dietary VD levels...