Rumen Microbiome and Metabolome of High and Low Residual Feed Intake Angus Heifers

作者：Yue Liu, Hao Wu, Wanbao Chen, Chang Liu, Qingxiang Meng, Zhenming Zhou · 发表于：Frontiers in Veterinary Science · 年份：2022 · DOI：10.3389/fvets.2022.812861 · 被引用次数：55 · 研究领域：Ruminant Nutrition and Digestive Physiology、Gout, Hyperuricemia, Uric Acid、Gut microbiota and health

Feed cost is the greatest expense during cattle production; therefore, reducing it is critical to increasing producer profits. In ruminants, the microbial population is important to nutrient digestion and absorption in the rumen. The objective of this study was to investigate the relationships among rumen bacteria, rumen metabolites, and the residual feed intake (RFI) phenotype of beef cattle. Twelve Angus heifers were selected to be sampled and divided into high RFI (HRFI; n = 6) group and low RFI (LRFI; n = 6) group according to their RFI classification determined during the feedlot-finishing period. After the ruminal liquid samples were collected at slaughter, Illumina MiSeq sequencing of the 16S rRNA V3-V4 region and liquid chromatography-mass spectrometry (LC-MS) were performed to determine their bacterial composition and metabolites, respectively. At the phylum level, the relative abundance of Proteobacteria was higher in the LRFI group than in the HRFI group ( P < 0.01). At the family level, the relative abundances of Rikenellaceae ( P < 0.01), Ruminococcaceae, Bacteroidales_S24-7_group , and Lachnospiraceae ( P < 0.05) were significantly higher in the LRFI group. At the genus level, the relative abundances of Rikenellaceae_RC9_gut_group and Ruminiclostridium_1 were higher in the LRFI group ( P < 0.01), as were the relative abundances of norank_f__Bacteroidales_S24-7_group, Lachnospiraceae_ND3007_group , and Lachnospiraceae_NK3A20_group ( P ...