Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction

作者：Daya Marasini, Melissa Whaley, Laurel T. Jenkins, Fang Hu, Wenxin Jiang, Nadav Topaz, Alex Chen, Susanna Schmink, Jennifer D. Thomas, Brian H. Harcourt, Henju Marjuki, Xin Wang · 发表于：Journal of Clinical Microbiology · 年份：2022 · DOI：10.1128/jcm.02111-21 · 被引用次数：10 · 研究领域：Bacterial Infections and Vaccines、Pneumonia and Respiratory Infections、Infective Endocarditis Diagnosis and Management

, Hi-abf, and Hi-cde were 100%. Triplex serotyping assays also showed 100% agreement for each serotype compared to their corresponding monoplex serotyping assay. These highly sensitive and specific drt-PCR assays do not require DNA extraction and thereby reduce the time, cost, and handling required to process CSF specimens. Furthermore, triplex drt-PCR assays combine the detection of three serotypes in a single reaction, further improving testing efficiency, which is critical for laboratories that process high volumes of Hi specimens for surveillance and diagnostic purposes.