Mussel-inspired extracellular matrix-mimicking hydrogel scaffold with high cell affinity and immunomodulation ability for growth factor-free cartilage regeneration

作者：Donglin Gan, Yanan Jiang, Yuelin Hu, Xiao Wang, Qiguang Wang, Kefeng Wang, Chaoming Xie, Lu Han, Xiong Lu · 发表于：Journal of Orthopaedic Translation · 年份：2022 · DOI：10.1016/j.jot.2022.02.006 · 被引用次数：53 · 研究领域：Osteoarthritis Treatment and Mechanisms、Hydrogels: synthesis, properties, applications、Tissue Engineering and Regenerative Medicine

Background: Injury to articular cartilage cause certain degree of disability due to poor self-repair ability of cartilage tissue. To promote cartilage regeneration, it is essential to develop a scaffold that properly mimics the native cartilage extracellular matrix (ECM) in the aspect of compositions and functions. Methods: A mussel-inspired strategy was developed to construct an ECM-mimicking hydrogel scaffold by incorporating polydopamine-modified hyaluronic acid (PDA/HA) complex into a dual-crosslinked collagen (Col) matrix for growth factor-free cartilage regeneration. The adhesion, proliferation, and chondrogenic differentiation of cells on the scaffold were examined. A well-established full-thickness cartilage defect model of the knee in rabbits was used to evaluated the efficacy and functionality of the engineered Col/PDA/HA hydrogel scaffold. Results: The PDA/HA complex incorporated-hydrogel scaffold with catechol moieties exhibited better cell affinity than bare negatively-charged HA incorporated hydrogel scaffold. In addition, the PDA/HA complex endowed the scaffold with immunomodulation ability, which suppressed the expression of inflammatory cytokines and effectively activated the polarization of macrophages toward M2 phenotypes. The in vivo results revealed that the mussel-inspired Col/PDA/HA hydrogel scaffold showed strong cartilage inducing ability to promote cartilage regeneration. Conclusions: The PDA/HA complex-incorporated hydrogel scaffold overcame the cel...