Establishment of PLAFMCi007-A, an induced pluripotent stem cell line, from peripheral blood mononuclear cells (PBMCs) of a healthy adult woman

作者：Meihan Shi, Shunlai Shang, Yunzhao Yang, Qinggang Li, Xue-Yuan Bai · 发表于：Stem Cell Research · 年份：2022 · DOI：10.1016/j.scr.2022.102760 · 被引用次数：2 · 研究领域：Pluripotent Stem Cells Research、Renal and related cancers、CRISPR and Genetic Engineering

Induced pluripotent stem cell (iPSC) lines for studies investigating many diseases can be established from peripheral blood mononuclear cells; here, an iPSC line was established from CD34+ cells isolated from the peripheral blood of a healthy woman. The cells were electrotransfected with three different recombinant plasmids to generate a normal-karyotype iPSC line that expresses characteristic surface markers and other pluripotent stem cell genes and can differentiate into all three germ layers in vivo. These newly established iPSC lines, a normal human cell line, can serve as a control line in studies investigating the pathogenesis of various diseases and meet the conditions for organoid studies.