In situ preparation of alendronate-loaded ZIF-8 nanoparticles on electrospun nanofibers for accelerating early osteogenesis in osteoporosis

作者：Mohammed A. Al-Baadani, Lihua Xu, Kendrick Hii Ru Yie, Anba Sun, Xue Gao, Kexin Cai, Bilal A. Al-Shaaobi, Abdullrahman M. Al-Bishari, Lei Cai, Xinkun Shen, Jinsong Liu, Pingping Ma · 发表于：Materials & Design · 年份：2022 · DOI：10.1016/j.matdes.2022.110596 · 被引用次数：49 · 研究领域：Bone Tissue Engineering Materials、Graphene and Nanomaterials Applications、Electrospun Nanofibers in Biomedical Applications

Applying guided bone regeneration membrane (GBRm) to treat bone defects in osteoporotic patients remains a challenge, as they cannot restore the fundamental balance between osteoblasts and osteoclasts, resulting in the ineffectiveness of osteoporotic bone injury treatment. Herein, a multifunctional polycaprolactone/gelatin (PG)-blended membrane embedded with alendronate-loaded zeolitic imidazolate framework-8 (Aln/Zif-8) nanoparticles was fabricated by electrospun technique and a simple solution-phase synthesis. Surface characterizations confirmed the successful synthesis of PG/Aln-Zif-8 nanofibers endowed with excellent mechanical strength. The release kinetics analysis showed the synergistic effects of the coordination bonds among Aln, zinc ions (Zn 2+ ), and organic ligands played a critical role in regulating the initial burst release and delayed release of Aln and Zn 2+ , respectively. The in vitro results of MC3T3-E1 and RAW264.3 cells demonstrated that the PG/Aln-Zif-8 membranes possessed superior anti-osteoporosis and osteoinductive properties. Furthermore, the PG/Aln-Zif-8 samples also exhibited significant bactericidal properties against both Staphylococcus aureus and Escherichia coli . The in vivo results further confirmed that PG/Aln-Zif-8 membranes had superior osteoinduction abilities, contributing to the accelerated osseointegration in the osteoporosis model. Altogether, these new bioinspired surface modifications highlight the promising alternative for fabrica...