GPR65 promotes intestinal mucosal Th1 and Th17 cell differentiation and gut inflammation through downregulating NUAK2

作者：Ritian Lin, Wei Wu, Huimin Chen, Han Gao, Xiaohan Wu, Gengfeng Li, Qiong He, Huiying Lu, Mingming Sun, Zhanju Liu · 发表于：Clinical and Translational Medicine · 年份：2022 · DOI：10.1002/ctm2.771 · 被引用次数：58 · 研究领域：Immune Response and Inflammation、Inflammatory Bowel Disease、T-cell and B-cell Immunology

Abstract G protein‐coupled receptor 65 (GPR65), a susceptibility gene for inflammatory bowel diseases (IBD), has been identified to promote Th17 cell pathogenicity and induce T cell apoptosis. However, the potential role of GPR65 in modulating CD4 + T cell immune responses in the pathogenesis of IBD stills not entirely understood. Here, we displayed that GPR65 expression was increased in inflamed intestinal mucosa of IBD patients and positively associated with disease activity. It was expressed in CD4 + T cells and robustly upregulated through the TNF‐α‐caspase 3/8 signalling pathway. Ectopic expression of GPR65 significantly promoted the differentiation of peripheral blood (PB) CD4 + T cells from IBD patients and HC to Th1 and Th17 cells in vitro. Importantly, conditional knockout of Gpr65 in CD4 + T cells ameliorated trinitrobenzene sulfonic acid (TNBS)‐induced acute murine colitis and a chronic colitis in Rag1 –/– mice reconstituted with CD45RB high CD4 + T cells in vivo, characterised by attenuated Th1 and Th17 cell immune response in colon mucosa and decreased infiltration of CD4 + T cells, neutrophils and macrophages. RNA‐seq analysis of Gpr65 ΔCD4 and Gpr65 flx/flx CD4 + T cells revealed that NUAK family kinase 2 ( Nuak2 ) acts as a functional target of Gpr65 to restrict Th1 and Th17 cell immune response. Mechanistically, GPR65 deficiency promoted NUAK2 expression via the cAMP‐PKA‐C‐Raf‐ERK1/2‐LKB1‐mediated signalling pathway. Consistently, silencing of Nuak2 facilitat...