Host‐induced silencing of a nematode chitin synthase gene enhances resistance of soybeans to both pathogenic Heterodera glycines and Fusarium oxysporum

作者：Lingan Kong, Xue Shi, Deng Chen, Nan Yang, Changfa Yin, Jun Yang, Gaofeng Wang, Wenkun Huang, Huan Peng, Deliang Peng, Shiming Liu · 发表于：Plant Biotechnology Journal · 年份：2022 · DOI：10.1111/pbi.13808 · 被引用次数：28 · 研究领域：Nematode management and characterization studies、Legume Nitrogen Fixing Symbiosis、Plant-Microbe Interactions and Immunity

Soybean cyst nematode (SCN), Heterodera glycines, is a devastating pathogen in soybean worldwide, causing huge yield losses annually. Host-induced gene silencing (HIGS) has shown potentials to control plant parasitic nematodes by generating transgenic plants carrying hairpin constructs against nematode target genes (Chaudhary et al., 2019; Li et al., 2010; Mani et al., 2020; Shivakumara et al., 2017; Tian et al., 2016). Chitin synthesized by chitin synthase (Chs) is present in fungi and nematodes but absent in plants and vertebrate animals and is a target in Magnaporthe oryzae and Fusarium graminearum for designing new fungicides and developing novel resistant varieties by HIGS (Cheng et al., 2015; Kong et al., 2012). Here, we mainly aimed to develop heritable SCN CHS-HIGS soybeans conferring enhanced SCN resistance. Meanwhile, the developed HIGS soybeans were tested for resistance to fungus Fusarium oxysporum, which causes soybean Fusarium wilt disease. First, the sole CHS gene (SCN-CHS) with 3984 bp (GenBank Acc. No.: OK149168) was cloned from SCN HG Type 1.2.3.5.7 (race 4, SCN4). SCN-Chs contained a typical chitin synthase catalytic domain (Chs) and seven transmembrane domains (TMs) (Figure 1a). qRT-PCR analysis indicated that SCN-CHS was highly expressed at egg stage when compared to other developmental stages (Figure 1b). A 420 bp cDNA fragment of SCN-CHS catalytic region positioned at 1936–2355 bp was used to generate transgenic HIGS soybeans employing ‘Jack’ as wild-ty...