Effect of naturally derived surgical hemostatic materials on the proliferation of A549 human lung adenocarcinoma cells

作者：Wei‐Dong Lü, Yizhi Liu, Yanqi Yang, Zhigang Liu, Kun Zhao, Jianrong Lu, Guangyan Lei, Yiyu Wang, Lin Cai, Ruifang Sun · 发表于：Materials Today Bio · 年份：2022 · DOI：10.1016/j.mtbio.2022.100233 · 被引用次数：11 · 研究领域：Hemostasis and retained surgical items、Wound Healing and Treatments、Electrospun Nanofibers in Biomedical Applications

Hemostatic materials are generally applied in surgical operations for cancer, but their effects on the growth and recurrence of tumors are unclear. Herein, three commonly used naturally derived hemostatic materials, gelatin sponge, Surgicel (oxidized regenerated cellulose), and biopaper (mixture of sodium hyaluronate and carboxymethyl chitosan), were cocultured with A549 human lung adenocarcinoma cells in vitro. Furthermore, the performance of hemostatic materials and the tumorigenicity of the materials with A549 cells were observed after subcutaneous implantation into BALB/c mice. The in vitro results showed that biopaper was dissolved quickly, with the highest cell numbers at 2 and 4 days of culture. Gelatin sponges retained their structure and elicited the least cell infiltration during the 2- to 10-day culture. Surgicel partially dissolved and supported cell growth over time. The in vivo results showed that biopaper degraded rapidly and elicited an acute Th1 lymphocyte reaction at 3 days after implantation, which was decreased at 7 days after implantation. The gelatin sponge resisted degradation and evoked a hybrid M1/M2 macrophage reaction at 7–21 days after implantation, and a protumor M2d subset was confirmed. Surgicel resisted early degradation and caused obvious antitumor M2a macrophage reactions. Mice subjected to subcutaneous implantation of A549 cells and hemostatic materials in the gelatin sponge group had the largest tumor volumes and the shortest overall surviv...