A defective lysophosphatidic acid-autophagy axis increases miscarriage risk by restricting decidual macrophage residence

作者：Hui‐Li Yang, Zhen‐Zhen Lai, Jiawei Shi, Wen‐Jie Zhou, Jie Mei, Jiang‐Feng Ye, Tao Zhang, Jian Wang, Jian‐Yuan Zhao, Da‐Jin Li, Ming‐Qing Li · 发表于：Autophagy · 年份：2022 · DOI：10.1080/15548627.2022.2039000 · 被引用次数：89 · 研究领域：Reproductive System and Pregnancy、Pregnancy and preeclampsia studies、Endometriosis Research and Treatment

Massive infiltrated and enriched decidual macrophages (dMφ) have been widely regarded as important regulators of maternal-fetal immune tolerance and trophoblast invasion, contributing to normal pregnancy. However, the characteristics of metabolic profile and the underlying mechanism of dMφ residence remain largely unknown. Here, we observe that dMφ display an active glycerophospholipid metabolism. The activation of ENPP2-lysophosphatidic acid (LPA) facilitates the adhesion and retention, and M2 differentiation of dMφ during normal pregnancy. Mechanistically, this process is mediated through activation of the LPA receptors (LPAR1 and PPARG/PPARγ)-DDIT4-macroautophagy/autophagy axis, and further upregulation of multiple adhesion factors (e.g., cadherins and selectins) in a CLDN7 (claudin 7)-dependent manner. Additionally, poor trophoblast invasion and placenta development, and a high ratio of embryo loss are observed in Enpp2±, lpar1−/− or PPARG-blocked pregnant mice. Patients with unexplained spontaneous abortion display insufficient autophagy and cell residence of dMφ. In therapeutic studies, supplementation with LPA or the autophagy inducer rapamycin significantly promotes dMφ autophagy and cell residence, and improves embryo resorption in Enpp2± and spontaneous abortion mouse models, which should be dependent on the activation of DDIT4-autophagy-CLDN7-adhesion molecules axis. This observation reveals that inactivation of ENPP2-LPA metabolism and insufficient autophagy of dM...