Exploration of Potential Roles of m5C-Related Regulators in Colon Adenocarcinoma Prognosis

作者：Yuancheng Huang, Chaoyuan Huang, Xiaotao Jiang, Yanhua Yan, Kunhai Zhuang, Fengbin Liu, Peiwu Li, Yi Wen · 发表于：Frontiers in Genetics · 年份：2022 · DOI：10.3389/fgene.2022.816173 · 被引用次数：18 · 研究领域：RNA modifications and cancer、RNA Research and Splicing、Cancer-related molecular mechanisms research

Objectives: The purpose of this study was to investigate the role of 13 m 5 C-related regulators in colon adenocarcinoma (COAD) and determine their prognostic value. Methods: Gene expression and clinicopathological data were obtained from The Cancer Genome Atlas (TCGA) datasets. The expression of m 5 C-related regulators was analyzed with clinicopathological characteristics and alterations within m 5 C-related regulators. Subsequently, different subtypes of patients with COAD were identified. Then, the prognostic value of m 5 C-related regulators in COAD was confirmed via univariate Cox regression and least absolute shrinkage and selection operator (LASSO) Cox regression analyses. The prognostic value of risk scores was evaluated using the Kaplan-Meier method, receiver operating characteristic (ROC) curve. The correlation between the two m 5 C-related regulators, risk score, and clinicopathological characteristics were explored. Additionally, Gene Set Enrichment Analysis (GSEA), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and Gene Ontology (GO) analysis were performed for biological functional analysis. Finally, the expression level of two m 5 C-related regulators in clinical samples and cell lines was detected by quantitative reverse transcription-polymerase chain reaction and through the Human Protein Atlas database. Results: m 5 C-related regulators were found to be differentially expressed in COAD with different clinicopathological features. We observed a hig...