A Primer for Single-Cell Sequencing in Non-Model Organisms

作者：James M. Alfieri, Guosong Wang, Michelle M. Jonika, C. A. Gill, Heath Blackmon, Giridhar Athrey · 发表于：Genes · 年份：2022 · DOI：10.3390/genes13020380 · 被引用次数：26 · 研究领域：Single-cell and spatial transcriptomics、Cell Image Analysis Techniques、Evolution and Genetic Dynamics

Single-cell sequencing technologies have led to a revolution in our knowledge of the diversity of cell types, connections between biological levels of organization, and relationships between genotype and phenotype. These advances have mainly come from using model organisms; however, using single-cell sequencing in non-model organisms could enable investigations of questions inaccessible with typical model organisms. This primer describes a general workflow for single-cell sequencing studies and considerations for using non-model organisms (limited to multicellular animals). Importantly, single-cell sequencing, when further applied in non-model organisms, will allow for a deeper understanding of the mechanisms between genotype and phenotype and the basis for biological variation.