A One-Step Electropolymerized Biomimetic Polypyrrole Membrane-Based Electrochemical Sensor for Selective Detection of Valproate

作者：Yuyang Yuan, Tianyu Li, Zhichao Ye, Yuyao Feng, Zhe Chen, Yusen Wang, Yiqiao Sun, Haoyu Wu, Zhaodong Yang, Yifan Wang, Yiran Zhang, Liquan Huang, Bo Liang · 发表于：Frontiers in Bioengineering and Biotechnology · 年份：2022 · DOI：10.3389/fbioe.2022.851692 · 被引用次数：17 · 研究领域：Pharmacological Effects and Toxicity Studies、Bipolar Disorder and Treatment、Epilepsy research and treatment

Bipolar disorder is a chronic mental disease with a heavy social and economic burden that causes extreme mood swings in patients. Valproate is a first-line drug for bipolar disorder patients to stabilize their daily mood. However, an excessive amount of valproate in the blood could induce severe adverse effects, which necessitates the monitoring of blood valproate levels for patients. Here, we developed an innovative electrochemical sensor for selective and simple detection of valproate based on a molecularly imprinted polymer membrane via one-step electropolymerization. Gold nanoparticles were electrochemically modified to the screen-printed electrode under the selective membrane to enhance its conductivity and stability. The successfully fabricated biosensor was characterized by scanning electron microscopy, cyclic voltammetry, and differential pulse voltammetry methods. The binding of the target molecules to the valproate-customized biomimetic polypyrrole membrane blocks cavities in the membrane and alters its electric properties, which can be detected as a decrease in the peak current by differential pulse voltammetry method. The peak current change presents a great log-linear response to the valproate concentration around the therapeutic window. The limit of detection of this method was 17.48 μM (LOD, S/N = 3) and the sensitivity was 31.86 μM μA −1 . Furthermore, the biosensors exhibited both satisfying specificity with the interference of other psychological pharmaceuti...