Viral Metagenomics Analysis of Rodents From Two Border Provinces Located in Northeast and Southwest China

作者：Teng Zhao, Yun-qi Dang, Wang Ao-Nan, Heting Gao, Hengduan Zhang, Dan Xing, Boqi Li, Yong-jiu Li, Zhu Liu, Chunxiao Li · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2021.701089 · 被引用次数：14 · 研究领域：Viral Infections and Vectors、Zoonotic diseases and public health、Mosquito-borne diseases and control

Objective Wild animal pathogen surveillance will help to understand the next possible pandemic in advance. Rodents, which have close contact with humans, are generally regarded as a key factor for zoonotic disease control. Given the variation in rodent virus composition in diverse ecologies, we conducted a study on the viral infection of rodents of diverse species in different typical environments of Heilongjiang and Yunnan Provinces, located in northeastern and southwestern China, respectively. Methods Viral metagenomics sequencing and bioinformatic analysis were performed to determine the different distributions of rodent-borne viruses in typical environments of Heilongjiang and Yunnan Provinces, China. After viral culture and PCR confirmation, genomic and phylogenetic quantitative analysis was performed on the detected hantaviruses (HVs) and Beilong viruses (BeiVs). Results Nineteen rodents from three species and 35 rodents from five species of rodents were collected from Heilongjiang and Yunnan Provinces, respectively. Although the number and number of species of rodents trapped in the northeast were fewer than those in the southwest, viruses annotated from rodents in Heilongjiang were more diverse than those in Yunnan. Rodents carried 22 virus families in Heilongjiang and 13 families in Yunnan. Sequences assembled from Rattus norvegicus were annotated to the M, L, and S segments of HV, and all were clustered within the Seoul-type hantavirus (SEOV). There were 2 (R81Q, S6...