Transcriptome reveals insights into biosynthesis of ginseng polysaccharides

作者：Xiaoxue Fang, Huaying Wang, Xinteng Zhou, Jing Zhang, Hongxing Xiao · 发表于：Research Square · 年份：2022 · DOI：10.21203/rs.3.rs-1357165/v1 · 被引用次数：2 · 研究领域：Ginseng Biological Effects and Applications、Microbial Metabolites in Food Biotechnology、Polysaccharides and Plant Cell Walls

Abstract Background: Ginseng polysaccharides, as an important active ingredient, has been used to treat various diseases. Nevertheless, the biosynthesis of ginseng polysaccharides is poorly understood. To elucidate the biosynthesis mechanism of ginseng polysaccharides, the transcriptome analysis and combined polysaccharide content determination were performed on the roots, stems and leaves collected from four cultivars of ginseng. Results: The results indicated that the total contents of nine monosaccharides were highest in the roots. Moreover, the total content of nine monosaccharides in the roots of the four ginseng cultivars were different, but were similar in stems and leaves. Glucose was the most component of all monosaccharides. In total, 19 potential enzymes for the synthesis of ginseng polysaccharides were identified, which 17 enzymes were significantly associated with polysaccharides content. Among these genes, phosphoglucomutase (PGM), glucose-6-phosphate isomerase (GPI), UTP--glucose-1-phosphate uridylyltransferase (UGP2), fructokinase (scrK), mannose-1-phosphate guanylyltransferase (GMPP), phosphomannomutase (PMM), UDP-glucose 4-epimerase (GALE), beta-fructofuranosidase (sacA) and sucrose synthase (SUS) were correlated with MYB, AP2/ERF, bZIP and NAC transcription factors. These transcription factors may regulate the expression of genes involved in ginseng polysaccharides synthesis. Conclusions: Our findings could provide an insight for a better understanding of t...