Physio-Morphological, Biochemical and Transcriptomic Analyses Provide Insights Into Drought Stress Responses in Mesona chinensis Benth

作者：Danfeng Tang, Changqian Quan, Lin Yang, Kunhua Wei, Shuangshuang Qin, Ying Liang, Wei Fan, Jianhua Miao · 发表于：Frontiers in Plant Science · 年份：2022 · DOI：10.3389/fpls.2022.809723 · 被引用次数：43 · 研究领域：Plant Stress Responses and Tolerance、Plant Gene Expression Analysis、Plant Molecular Biology Research

Drought stress affects the normal growth and development of Mesona chinensis Benth (MCB), which is an important medicinal and edible plant in China. To investigate the physiological and molecular mechanisms of drought resistance in MCB, different concentrations of polyethylene glycol 6000 (PEG6000) (0, 5, 10, and 15%) were used to simulate drought conditions in this study. Results showed that the growth of MCB was significantly limited under drought stress conditions. Drought stress induced the increases in the contents of Chla, Chlb, Chla + b, soluble protein, soluble sugar, and soluble pectin and the activities of superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (TAC), hydrogen peroxide (H 2 O 2 ), and malondialdehyde (MDA). Transcriptome analysis revealed 3,494 differentially expressed genes (DEGs) (1,961 up-regulated and 1,533 down-regulated) between the control and 15% PEG6000 treatments. These DEGs were identified to be involved in the 10 metabolic pathways, including “plant hormone signal transduction,” “brassinosteroid biosynthesis,” “plant–pathogen interaction,” “MAPK signaling pathway-plant,” “starch and sucrose metabolism,” “pentose and glucuronate interconversions,” “phenylpropanoid biosynthesis,” “galactose metabolism,” “monoterpenoid biosynthesis,” and “ribosome.” In addition, transcription factors (TFs) analysis showed 8 out of 204 TFs, TRINITY_DN3232_c0_g1 [ ABA-responsive element ( ABRE ) -binding transcription factor1, AREB1 ], TRINITY_...