Cell types of origin of the cell-free transcriptome

作者：Sevahn K. Vorperian, Mira N. Moufarrej, Overall Project Direction and Coordination, Robert C. Jones, Jim Karkanias, Mark A. Krasnow, Angela Oliveira Pisco, Stephen R. Quake, Julia Salzman, Nir Yosef, Donor Recruitment, Bryan Bulthaup, Phillip Brown, William Harper, Marisa Hemenez, Ravikumar Ponnusamy, Ahmad Salehi, Bhavani A. Sanagavarapu, Eileen Spallino, Surgeons, Ksenia A. Aaron, Waldo Concepcion, James M. Gardner, Burnett S. Kelly, Nikole Neidlinger, Zifa Wang, Logistical coordination, Sheela Crasta, Saroja Kolluru, Maurizio Morri, Serena Y. Tan, Kyle J. Travaglini, Chenling Xu, Organ Processing, Marcela Alcántara‐Hernández, Nicole Almanzar, Jane Antony, Benjamin Beyersdorf, Deviana Burhan, Kruti Calcuttawala, Matthew M. Carter, Charles K. F. Chan, Charles Chang, Stephen Chang, Alex Colville, Rebecca N. Culver, Ivana Cvijović, Gaetano D’Amato, Camille Ezran, Francisco X. Galdos, Astrid Gillich, William R. Goodyer, Yan Hang, Alyssa Hayashi, Sahar Houshdaran, Xianxi Huang, Juan C. Irwin, SoRi Jang, Júlia Vallvé-Juanico, Aaron M. Kershner, Soochi Kim, Bernhard Kiss, William Kong, Maya E. Kumar, Angera H. Kuo, Rebecca Leylek, Baoxiang Li, Gabriel B. Loeb, Wan-Jin Lu, Sruthi Mantri, Maxim Markovic, Patrick L. McAlpine, Antoine de Morrée, Karim Mrouj, Shravani Mukherjee, Tyler Muser, Patrick Neuhöfer, Thi D. Nguyen, Kimberly Perez, Ragini Phansalkar, Nazan Puluca, Zhen Qi, Poorvi Rao, Hayley M. Raquer-McKay, Nicholas Schaum, Bronwyn Scott, Bobak Seddighzadeh, Joe M. Segal, Sushmita Sen, Shaheen S. Sikandar, Sean P. Spencer, L.C. Steffes, Varun Ramanan Subramaniam, Aditi Swarup, Michael Swift, Will Van Treuren, Emily Trimm, Stefan Veizades, Sivakamasundari Vijayakumar, Kim Chi Vo, Sevahn K. Vorperian, Wanxin Wang, H Weinstein, Juliane Winkler, Ting-Hsuan Wu, Jamie Xie, Andrea R. Yung, Yue Zhang, Sequencing, Angela M. Detweiler, Honey Mekonen, Norma Neff, Rene Sit, Michelle Tan, Jia Yan, Histology, Gregory R. Bean, Vivek Charu, Erna Forgó, Brock A. Martin, Michael G. Ozawa, Oscar Silva, Angus Toland, Venkata N. P. Vemuri, Data Analysis, Shaked Afik, Kyle Awayan, Rob Bierman, Olga Botvinnik, Ashley Byrne, Michelle Chen, Roozbeh Dehghannasiri, Adam Gayoso, Alejandro A. Granados, Qiqing Li, Gita Mahmoudabadi, Aaron McGeever, Julia Olivieri, Madeline Park, Neha Ravikumar, Geoff Stanley, Weilun Tan, Alexander J. Tarashansky, Rohan Vanheusden, Peter Wang, Sheng Wang, Galen Xing, Chenling Xu, Nir Yosef, Expert Cell Type Annotation, Rebecca N. Culver, Les Dethlefsen, Po-Yi Ho, Shixuan Liu, Jonathan S. Maltzman, Ross J. Metzger, Koki Sasagawa, Rahul Sinha, Hanbing Song, Bruce Wang, Tissue Expert Principal Investigators, Steven E. Artandi, Philip A. Beachy, Michael F. Clarke, Linda C. Giudice, Franklin W. Huang, Kerwyn Casey Huang, Juliana Idoyaga, Seung K. Kim, Christin S. Kuo, Patricia K. Nguyen, Thomas A. Rando, Kristy Red-Horse, Jeremy F. Reiter, David A. Relman, Justin L. Sonnenburg, Albert Y. Wu, Sean M. Wu, Tony Wyss‐Coray, Stephen R. Quake · 发表于：Nature Biotechnology · 年份：2022 · DOI：10.1038/s41587-021-01188-9 · 被引用次数：106 · 研究领域：Single-cell and spatial transcriptomics、Cancer Genomics and Diagnostics、Cancer-related molecular mechanisms research

Cell-free RNA from liquid biopsies can be analyzed to determine disease tissue of origin. We extend this concept to identify cell types of origin using the Tabula Sapiens transcriptomic cell atlas as well as individual tissue transcriptomic cell atlases in combination with the Human Protein Atlas RNA consensus dataset. We define cell type signature scores, which allow the inference of cell types that contribute to cell-free RNA for a variety of diseases.