Skeletal Muscle Expression of Actinin-3 (ACTN3) in Relation to Feed Efficiency Phenotype of F2Bos indicus - Bos taurus Steers

作者：Robert N. Vaughn, Kelli J. Kochan, Aline K. Torres, ‬Min Du, David G. Riley, C. A. Gill, A. D. Herring, James O. Sanders, Penny K. Riggs · 发表于：Frontiers in Genetics · 年份：2022 · DOI：10.3389/fgene.2022.796038 · 被引用次数：3 · 研究领域：Genetic and phenotypic traits in livestock、Genetics and Physical Performance、Genetic Mapping and Diversity in Plants and Animals

In this study, actinin-3 ( ACTN3 ) gene expression was investigated in relation to the feed efficiency phenotype in Bos indicus - Bos taurus crossbred steers. A measure of relative feed efficiency based on residual feed intake relative to predictions from the NRC beef cattle model was analyzed by the use of a mixed linear model that included sire and family nested within sire as fixed effects and age, animal type, sex, condition, and breed as random effects for 173 F 2 Nellore-Angus steers. Based on these residual intake observations, individuals were ranked from most efficient to least efficient. Skeletal muscle samples were analyzed from 54 steers in three groups of 18 (high efficiency, low efficiency, and a statistically average group). ACTN3 , which encodes a muscle-specific structural protein, was previously identified as a candidate gene from a microarray analysis of RNA extracted from muscle samples obtained from a subset of steers from each of these three efficiency groups. The expression of ACTN3 was evaluated by quantitative reverse transcriptase PCR analysis. The expression of ACTN3 in skeletal muscle was 1.6-fold greater in the inefficient steer group than in the efficient group ( p = 0.007). In addition to expression measurements, blocks of SNP haplotypes were assessed for breed or parent of origin effects. A maternal effect was observed for ACTN3 inheritance, indicating that a maternal B. indicus block conferred improved residual feed efficiency relative to the ...