Inhalable antibiotic resistomes emitted from hospitals: metagenomic insights into bacterial hosts, clinical relevance, and environmental risks

作者：Dong Wu, Ling Jin, Jiawen Xie, Hang Liu, Jue Zhao, Dan Ye, Xiangdong Li · 发表于：Microbiome · 年份：2022 · DOI：10.1186/s40168-021-01197-5 · 被引用次数：124 · 研究领域：Gut microbiota and health、Indoor Air Quality and Microbial Exposure、Pharmaceutical and Antibiotic Environmental Impacts

Abstract Background Threats of antimicrobial resistance (AMR) to human health are on the rise worldwide. Airborne fine particulate matter (PM 2.5 ), especially those emitted from hospitals, could serve as a substantial yet lesser-known environmental medium of inhalable antibiotic resistomes. A genome-centric understanding of the hosting bacterial taxa, mobility potential, and consequent risks of the resistomes is needed to reveal the health relevance of PM 2.5 -associated AMR from clinical settings. Results Compared to urban ambient air PM 2.5 , the hospital samples harbored nearly twice the abundance of antibiotic resistantance genes (ARGs, ~ 0.2 log 10 (ARGs/16S rRNA gene)) in the summer and winter sampled. The profiled resistome was closely correlated with the human-source-influenced (~ 30% of the contribution) bacterial community (Procrustes test, P < 0.001), reflecting the potential antibiotic-resistant bacteria (PARB), such as the human commensals Staphylococcus spp. and Corynebacterium spp. Despite the reduced abundance and diversity of the assembled metagenomes from summer to winter, the high horizontal transfer potential of ARGs, such as the clinically relevant bla OXA and bac A, in the human virulent PARB remained unaffected in the hospital air PM samples. The occurring patterns of β-lactam resistance genes and their hosting genomes in the studied hospital-emitting PM 2.5 were closely related to the in-ward β-lactam-resistant infections (SEM, std = 0.62, P < 0...