Microbial Characteristics and Genomic Analysis of an ST11 Carbapenem-Resistant Klebsiella pneumoniae Strain Carrying blaKPC−2 Conjugative Drug-Resistant Plasmid

作者：Lingyi Zeng, Jisheng Zhang, Kewang Hu, Jie Li, Jianmin Wang, Chengru Yang, Wan Huang, Lining Yin, Xiaoli Zhang · 发表于：Frontiers in Public Health · 年份：2022 · DOI：10.3389/fpubh.2021.809753 · 被引用次数：12 · 研究领域：Antibiotic Resistance in Bacteria、Bacterial Genetics and Biotechnology、Bacterial Identification and Susceptibility Testing

Background The sequence type 11 (ST11) carbapenem-resistant Klebsiella pneumoniae (CRKP) carrying bla KPC −2 has been widespread all over the world, and it has been reported frequently in China. The bla KPC −2 located on the mobile genetic element brings tremendous pressure to control the spread and outbreak of resistant bacteria. Whole-genome sequencing (WGS) technology can comprehensively and in-depth display the molecular characteristics of drug-resistant bacteria, providing a basis for evaluating the genetic diversity within the CRKP genome. Methods The ST11 CRKP in this study was collected in the intensive care unit of a major teaching hospital. PCR and Sanger sequencing confirmed the existence of bla KPC −2 . The AST-GN card and the microbroth dilution test were used for antimicrobial susceptibility testing. The transferability of plasmid was verified by a conjugation test. The whole genome is sequenced using the Illumina HiSeq short-read and Oxford Nanopore long-read sequencing technology. Results The studied strain was named CRKP63, which is a multi-drug resistance bacteria, which carries bla KPC −2 and bla SHV −182 . Its genome consists of a circular chromosome of 5,374,207 bp and an IncFII plasmid named pKPC-063001 of 359,625 bp. In the drug-resistant plasmid pKPC-063001, the key carbapenem resistance gene bla KPC −2 was located in the genetic context with insertion sequence IS Kpn27 upstream and IS Kpn6 downstream and bracketed by IS 26 . The three copies of the IS...