RETRACTED ARTICLE: Osteogenic differentiation of mesenchymal stem cells promotes c-Jun-dependent secretion of interleukin 8 and mediates the migration and differentiation of CD4+ T cells

作者：Ye Feng, Jinteng Li, Peitao Xu, Zhongyu Xie, Zheng Guan, Wenjie Liu, Guiwen Ye, Wenhui Yu, Jiajie Lin, Zepeng Su, Yunshu Che, Zhaoqiang Zhang, Peng Wang, Yanfeng Wu, Huiyong Shen · 发表于：Stem Cell Research & Therapy · 年份：2022 · DOI：10.1186/s13287-022-02735-0 · 被引用次数：47 · 研究领域：Mesenchymal stem cell research、Bone Metabolism and Diseases、Bone and Joint Diseases

Abstract Background The immune system and the skeletal system have complex interactions in the bone marrow and even in the joints, which has promoted the development of the concept of osteoimmunology. Some evidence has indicated that T cells and B cells contribute to the balance between the resorption and formation of bone. However, there has been little discussion on the regulation of CD4 + T lymphocytes by cells involved in bone metabolism. Mesenchymal stem cells (MSCs), which exert core functions related to immunoregulation and osteogenic differentiation, are crucial cells linked to both bone metabolism and the immune system. Previous studies have shown that the immunoregulatory capacity of MSCs changes following differentiation. However, it is still unclear whether the osteogenic differentiation of MSCs affects the migration and differentiation of CD4 + T cells. Methods MSCs were cultured in growth medium or osteogenic medium for 10 days and then cocultured with CD4+ T cells. CD4+ T cell migration and differentiation were detected by flow cytometry. Further, gene expression levels of specific cytokines were analyzed by quantitative real-time PCR and enzyme-linked immunosorbent assays. A Proteome Profiler Human XL Cytokine Array Kit was used to analyze supernatants collected from MSCs. Alizarin red S staining and Alkaline phosphatase assay were used to detect the osteogenic differentiation of MSCs. Results Here, we found that the migration of CD4 + T cells was elevated, an...