Multiplexed immunosensors for point-of-care diagnostic applications

作者：Bruno M. G. Rosa, Oluwatomi E. Akingbade, Xiaotong Guo, Laura Gonzalez‐Macia, Michael A. Crone, Loren P. Cameron, Paul S. Freemont, Kwang‐Leong Choy, Firat Güder, Eric M. Yeatman, David Sharp, Bing Li · 发表于：Biosensors and Bioelectronics · 年份：2022 · DOI：10.1016/j.bios.2022.114050 · 被引用次数：182 · 研究领域：Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection、Molecular Junctions and Nanostructures

Accurate, reliable, and cost-effective immunosensors are clinically important for the early diagnosis and monitoring of progressive diseases, and multiplexed sensing is a promising strategy for the next generation of diagnostics. This strategy allows for the simultaneous detection and quantification of multiple biomarkers with significantly enhanced reproducibility and reliability, whilst requiring smaller sample volumes, fewer materials, and shorter average analysis time for individual biomarkers than individual tests. In this opinionated review, we compare different techniques for the development of multiplexed immunosensors. We review the state-of-the-art approaches in the field of multiplexed immunosensors using electrical, electrochemical, and optical methods. The barriers that prevent translating this sensing strategy into clinics are outlined together with the potential solutions. We also share our vision on how multiplexed immunosensors will continue their evolution in the coming years.