Preparation of Puerarin Nanocrystals and Their Application on Rats with Myocardial Ischemic Injury Combined with Electroacupuncture-PC6 (Neiguan)

作者：Lingjuan Kong, Yejuan Jia, Tianyu Li, Yu Chen, Lijia Pan, Chun‐Sheng Jia · 发表于：Science of Advanced Materials · 年份：2021 · DOI：10.1166/sam.2021.4142 · 被引用次数：1 · 研究领域：Acupuncture Treatment Research Studies、Vagus Nerve Stimulation Research、Healthcare and Venom Research

The effect of electroacupuncture PC6 (Neiguan) combined with puerarin nanocrystal preparation (PUE-NP) on myocardial injury and autophagy in a rat model with myocardial ischemia was explored in this study. PUE-NPs were first prepared, and there was an analysis on the fourier transform infrared spectroscopy (FTIR), particle size distribution of nanocrystalline preparation, Zeta potential, and X-ray diffractometry (XRD) characterization, so as to analyze the difference in the in vitro cumulative release rate of PUE and PUE-NPs. Secondly, Sprague-Dawley (SD) rats were taken as the subjects investigated. Besides, they were divided randomly into a sham surgery group (Sham), a myocardial ischemia model group (Model), an electroacupuncture Neiguan acupoint pretreatment model group (EA+M), an electroacupuncture Neiguan acupoint pretreatment combined with PUE treatment model group (EA+PUE+M), and an acupuncture Neiguan point pretreatment combined with PUENPs treatment model group (EA+PUE-NPs+M). Then, the rats were treated with abdominal anesthesia, and their serums were extracted to detect the differences in serum creatine kinase MB isoenzyme (CK-MB) and lactate dehydrogenase (LDH) concentrations. After the rats were sacrificed, myocardial tissues were taken out, and the Western blot, hematoxylin-eosin (HE) staining, and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining were employed to detect apoptosis index, pathological changes of myocar...