Genetic Diversity and Genome Size Variability in the Russian Genebank Collection of Tea Plant [Camellia sinensis (L). O. Kuntze]

作者：Lidiia Samarina, A. O. Matskiv, Ruset M. Shkhalakhova, N. G. Koninskaya, Magda‐Viola Hanke, Henryk Flachowsky, Alexander N. Shumeev, Karina Manakhova, L. S. Malyukova, Shengrui Liu, Juanyan Zhu, М. V. Gvasaliya, V. I. Malyarovskaya, Alexey Ryndin, E. K. Pchikhachev, Stefanie Reim · 发表于：Frontiers in Plant Science · 年份：2022 · DOI：10.3389/fpls.2021.800141 · 被引用次数：20 · 研究领域：Genetic diversity and population structure、Plant Pathogens and Fungal Diseases、Plant and Fungal Interactions Research

The tea collection of the FRC SSC RAS (Sochi, Maykop in Russia) represents one of the northernmost germplasm comprising a number of locally derived cultivars and ɣ-irradiation mutants. The latter are often characterized by larger genome size, which may lead to better adaptation to biotic and abiotic stress. Such genotypes may be a valuable genetic resource for better adaptability to extreme environmental conditions, which could enable tea cultivation outside global growing regions. Microsatellite markers are often the best choice for genetic diversity analysis in genebank collections. However, their use in polyploid species is questionable because simple sequence repeat (SSR) allele dosage cannot be readily determined. Therefore, the efficiency of SSR and start codon targeted (SCoT) markers was investigated using 43 selected cultivars from the Russian genebank collection derived from mutant breeding and clonal selection. Previously, the increase in genome size was confirmed in 18 mutants within this collection. Despite the presence of polyploid tea genotypes, our study revealed higher efficiency of SSR markers than SCoT markers. Subsequent SSR analysis of the 106 genotypes in the Russian genebank collection revealed three distinct genetic clusters after STRUCTURE analysis. Greater genetic variation was observed within genetic clusters than between clusters, indicating low genetic variation between collections. Nevertheless, the northernmost tea collection exhibited a greater ...