Effects of Mycobacterium tuberculosis lineages and regions of difference (RD) virulence gene variation on tuberculosis recurrence

作者：Chuanjiang He, Xiang Cheng, Aihemaitijiang Kaisaier, Jiangli Wan, Sheng-Fang Luo, Jie Ren, Yin-Zhong Sha, Hongmei Peng, Ya-Hui Zhen, Wen Liu, Sujie Zhang, Jingran Xu, Aimin Xu · 发表于：Annals of Translational Medicine · 年份：2022 · DOI：10.21037/atm-21-6863 · 被引用次数：20 · 研究领域：Tuberculosis Research and Epidemiology、Mycobacterium research and diagnosis、Diagnosis and treatment of tuberculosis

Background: and TB relapse can provide basic data for the study of TB prevention and control. Methods: RD region were analyzed. The maximum likelihood method was implemented using IQ-TREE software. Logistic regression was used to analyze the relationship between lineage, RD region virulence gene variation, and patient relapse. Results: lineages: L2 (45.80%), L3 (32.90%), and L4 (21.30%). In relapsed patients, L2 (70.83%, 17/24) was significantly higher than the other lineages (29.17%, 7/24; P<0.05). Relapse was significantly correlated with L2 [odds ratio (OR) =3.505; 95% confidence interval (CI): 1.341-9.158; P=0.011]. In the virulence genes of the RD region, g.4357804 (T→G, OR =4.278; 95% CI: 1.594-11.481; P=0.004), g.4359653 (C→T, OR =3.356; 95% CI: 1.303-8.644; P=0.012), and g.2627618 (C→A, OR =2.676; 95% CI: 1.101-6.502; P=0.030) mutations were significantly associated with patient relapse. The mutation frequencies of g.4357804, g.4359653, and g.2627618 in L2 were significantly higher than those in the non-L2 group (P<0.05). Conclusions: Patients infected with L2 are more prone to relapse, and RD region virulence gene variation is an important factor for the strong pathogenicity and easy relapse after infection associated with L2.