Gene Identification, Evolution and Expression of <i>MCT</i> Gene in Mango (<i>Mangifera indica</i>)

作者：Jiangtao Gai, Peng Wang, Xiuxu Ye, Yeyuan Chen · 发表于：Plant Gene and Trait · 年份：2021 · DOI：10.5376/pgt.2021.12.0004 · 研究领域：Plant Molecular Biology Research

2-C-methyl- D -erythritol 4-phosphate cytidylyltransferase (MCT) is a key enzyme involved in the 2- C -methyl- D -erythritol-4-phosphate (MEP) pathway. In order to identify the MCT gene in mango, study the evolutionary route and expression pattern of  MCT  gene. In this study, with arabidopsis  MCT  gene as a reference, 14 MCT genes were searched from 12 plant genomes through the BLAST and HMMER. Then, we analyzed the conserved domains, phylogeny, and the expression of  MCT  gene in mango. The results showed that the MCT protein of mango contains a PF01128.19 domain, and the protein sequences of  MCT  gene is highly conserved. Phylogenetic analysis showed that the evolution of  MCT  genes were consistent with the evolutionary route of its species, the number of copies of the  MCT  genes was tightly controlled from algae to angiosperms. The results of expression analysis showed that the expression of  MCT  gene in the peels and flesh of developing mango were higher than that in the peels and flesh of mature mango in ‘guire-82’and ‘hongyu’. It is speculated that  MCT  gene play a more important role in the development of mango. These provide scientific basis for further elucidating the function of  MCT  gene in mango.