Single-Electrode Electrochemical System for the Visual and High-Throughput Electrochemiluminescence Immunoassay

作者：Fangxin Du, Zhiyong Dong, Yiran Guan, Abdallah M. Zeid, Di Ma, Jiachun Feng, Di Yang, Guobao Xu · 发表于：Analytical Chemistry · 年份：2022 · DOI：10.1021/acs.analchem.1c04709 · 被引用次数：91 · 研究领域：Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection、Advanced Biosensing Techniques and Applications

The electrochemiluminescence (ECL) immunoassay with its visual and high-throughput detection has received considerable attention in the past decade. However, the development of a facile and cost-effective ECL device is still a great challenge. Herein, a single-electrode electrochemical system (SEES) for the visual and high-throughput ECL immunoassay was developed. The SEES was designed by attaching a plastic sticker with multiple holes onto a single carbon ink screen-printed electrode based on a resistance-induced potential difference. Due to its excellent properties of adsorption and bioaffinity, the carbon ink screen-printed electrode is applied to immobilize antibodies. When cardiac troponin I (cTnI), a specific biomarker of acute myocardial infarction, is present, it will be captured by the immobilized cTnI antibodies on the electrode surface, inhibiting electron transfer, resulting in a decrease of the ECL intensity of the luminol–H 2 O 2 system. Using a smartphone as the detector, cTnI could be determined, ranging from 1 to 1000 ng mL –1, with a detection limit of 0.94 ng mL –1 . The SEES based on the carbon ink screen-printed electrode is characterized by its high simplicity, cost effectiveness, and user-friendliness compared with conventional three-electrode systems and bipolar electrochemical systems using electrode arrays and shows superior advantages over other immunoassay strategies, with the elimination of multistep assembling and labeling processes. What is more...