Resveratrol Treats UVB‐Induced Photoaging by Anti‐MMP Expression, through Anti‐Inflammatory, Antioxidant, and Antiapoptotic Properties, and Treats Photoaging by Upregulating VEGF‐B Expression

作者：Baining Cui, Yan Wang, Jiahui Jin, Zhen Yang, Ruoxi Guo, Xue Li, Lei Yang, Zheng Li · 发表于：Oxidative Medicine and Cellular Longevity · 年份：2022 · DOI：10.1155/2022/6037303 · 被引用次数：141 · 研究领域：Skin Protection and Aging、Genomics, phytochemicals, and oxidative stress、Photodynamic Therapy Research Studies

UVB exposure is one of the primary factors responsible for the development of photoaging, and the aim of this study was to investigate the mechanism involved in the photoprotective properties of resveratrol (RES) in UVB‐induced photoaging. Photoaging models of Hacat cells and ICR mice were established by UVB irradiation. The effect of RES on cell viability was then assessed using the MTT assay. The effect of RES on reactive oxygen species (ROS) production was detected through a fluorescent probe assay. The effect of RES on oxidized glutathione (GSSH) content, and superoxide dismutase (SOD) activity in photoaging Hacat cells, were measured separately, using kits. An enzyme‐linked immunosorbent assay (ELISA) was used to measure the effect of RES on IL‐6 secretion. The effect of VEGF‐B on RES photoprotection was examined through the RT‐qPCR method, after silencing VEGF‐B through siRNA transfection. For animal experiments, the relative water content of the skin of ICR mice was determined using the Corneometer CM825 skin moisture tester. Starting from the third week of the study, the back skin of photoaging ICR mice was photographed weekly using the TIVI700 camera, and the depth of skin wrinkles in photoaging ICR mice was also analyzed. The thickness of the epidermis in photoaging ICR mice was assessed by the hematoxylin‐eosin (HE) staining method. The content of collagen fibers in the skin dermis of photoaging ICR mice was measured by the Masson trichrome staining method. The con...