Single cell atlas for 11 non-model mammals, reptiles and birds

作者：Dongsheng Chen, Jian Sun, Jiacheng Zhu, Xiangning Ding, Tianming Lan, Xiran Wang, Weiying Wu, Zhihua Ou, Linnan Zhu, Peiwen Ding, Haoyu Wang, Lihua Luo, Rong Xiang, Xiaoling Wang, Jiaying Qiu, Shiyou Wang, Haimeng Li, Chaochao Chai, Langchao Liang, Fuyu An, Le Zhang, Lei Han, Yixin Zhu, Fei–Yue Wang, Yuting Yuan, Wendi Wu, Chengcheng Sun, Haorong Lu, Jihong Wu, Xinghuai Sun, Shenghai Zhang, Sunil Kumar Sahu, Ping Liu, Jun Xia, Lijing Zhang, Hạixia Chen, Dongming Fang, Yuying Zeng, Yiquan Wu, Ze-Hua Cui, Qian He, Sanjie Jiang, Xiaoyan Ma, Weimin Feng, Yan Xu, Yan Xu, Zhongmin Liu, Zhongmin Liu, Fang Chen, Fang Chen, Xin Jin, Wei Qiu, Li Yang, Yang Li, Xiumei Xing, Huanming Yang, Yanchun Xu, Yahong Liu, Yan Hua, YaHong Liu, Huan Liu, Xun Xu · 发表于：Nature Communications · 年份：2021 · DOI：10.1038/s41467-021-27162-2 · 被引用次数：75 · 研究领域：Single-cell and spatial transcriptomics、SARS-CoV-2 and COVID-19 Research、Respiratory viral infections research

The availability of viral entry factors is a prerequisite for the cross-species transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Large-scale single-cell screening of animal cells could reveal the expression patterns of viral entry genes in different hosts. However, such exploration for SARS-CoV-2 remains limited. Here, we perform single-nucleus RNA sequencing for 11 non-model species, including pets (cat, dog, hamster, and lizard), livestock (goat and rabbit), poultry (duck and pigeon), and wildlife (pangolin, tiger, and deer), and investigated the co-expression of ACE2 and TMPRSS2. Furthermore, cross-species analysis of the lung cell atlas of the studied mammals, reptiles, and birds reveals core developmental programs, critical connectomes, and conserved regulatory circuits among these evolutionarily distant species. Overall, our work provides a compendium of gene expression profiles for non-model animals, which could be employed to identify potential SARS-CoV-2 target cells and putative zoonotic reservoirs.