Comparison of NF-κB from the protists Capsaspora owczarzaki and Acanthoeca spectabilis reveals extensive evolutionary diversification of this transcription factor

作者：Leah M. Williams, Sainetra Sridhar, Jason Samaroo, Jada A. Peart, Ebubechi Adindu, Anvitha Addanki, Christopher J. DiRusso, Dana H. M. Alburi, Ludmila Anisimov, Aria Y. Armstrong, Sydney J. Badger, Elham Banaie, Joana Teixeira, Madeleine T. Billingsley, Anoush Calikyan, Yinxing Chen, Aidan B. Coia, Daniel Cutillo, Breanna R. Dooling, Parth P. Doshi, Kyra R. Dubinsky, Berta Escude Velasco, Jabari R. Evans, Jasmine Gordon, Huibo Guan, Spiro N. Haliotis, Niccolas T. Hood, Yen-Chun Huang, Wenjing Jiang, Isabelle Kreber, Ekin B. Kurak, Cheng-Che Lee, Tanner M. Lehmann, Savina J. W. Lin, Edward Liu, Kevin Liu, Yen‐Yu Liu, Alexandra L. Luther, Alexa A. Macgranaky-Quaye, Daniel J. Magat, Lauren E. Malsick, Parmida Masoudi, Parsida Masoudi, Chad R. H. Max, Ethan Z. McCaslin, Eleanor T. McGeary, Kathleen M. McLaughlin, Victoria S. A. Momyer, L. Murphy, Sonny V. Nguyen, Kareemah Ni, Leon Novak, Roberto Nunes Campos E. Santos, Yemi Osayame, Jun Bai Park Chang, Harshal M. Patel, Tony V. Pham, Sheila M. Phillips, Jhonathan Perea Piedrahita, Tricia L. Post, Rebecca A. Prather, Pauline I. Reck, Jaime Rodrı́guez, Kirquenique A. Rolle, Joseph A. Salzo, Kathryn M. Satko, Davis G. Settipane, Kara J. Sevola, Mithil V. Shah, Viktoriya Skidanova, Georgia M. Snyder, Rebecca J. Sprague, Ryan A. Stagg, Danielle Tong, Andreas A. Towers, Nicholas W. Turgiss, Natalie S. Wheeler, Ann Yung, Pablo J. Aguirre Carrión, Nahomie Rodríguez-Sastre, Trevor Siggers, Thomas D. Gilmore · 发表于：Communications Biology · 年份：2021 · DOI：10.1038/s42003-021-02924-2 · 被引用次数：8 · 研究领域：Protist diversity and phylogeny、Echinoderm biology and ecology、Parasitic Infections and Diagnostics

We provide a functional characterization of transcription factor NF-κB in protists and provide information about the evolution and diversification of this biologically important protein. We characterized NF-κB in two protists using phylogenetic, cellular, and biochemical techniques. NF-κB of the holozoan Capsaspora owczarzaki (Co) has an N-terminal DNA-binding domain and a C-terminal Ankyrin repeat (ANK) domain, and its DNA-binding specificity is more similar to metazoan NF-κB proteins than to Rel proteins. Removal of the ANK domain allows Co-NF-κB to enter the nucleus, bind DNA, and activate transcription. However, C-terminal processing of Co-NF-κB is not induced by IκB kinases in human cells. Overexpressed Co-NF-κB localizes to the cytoplasm in Co cells. Co-NF-κB mRNA and DNA-binding levels differ across three Capsaspora life stages. RNA-sequencing and GO analyses identify possible gene targets of Co-NF-κB. Three NF-κB-like proteins from the choanoflagellate Acanthoeca spectabilis (As) contain conserved Rel Homology domain sequences, but lack C-terminal ANK repeats. All three As-NF-κB proteins constitutively enter the nucleus of cells, but differ in their DNA-binding abilities, transcriptional activation activities, and dimerization properties. These results provide a basis for understanding the evolutionary origins of this key transcription factor and could have implications for the origins of regulated immunity in higher taxa.