Calcium imaging reveals depressive- and manic-phase-specific brain neural activity patterns in a murine model of bipolar disorder: a pilot study

作者：Min Chen, Hongjun Tian, Guoyong Huang, Tao Fang, Xiaodong Lin, Jianmin Shan, Ziyao Cai, Gaungdong Chen, Suling Chen, Ce Chen, Jing Ping, Langlang Cheng, Chunmian Chen, Jingjing Zhu, Feifei Zhao, Deguo Jiang, Chuanxin Liu, Guangchuan Huang, Chongguang Lin, Chuanjun Zhuo · 发表于：Translational Psychiatry · 年份：2021 · DOI：10.1038/s41398-021-01750-8 · 被引用次数：10 · 研究领域：Bipolar Disorder and Treatment、Tryptophan and brain disorders、Neuroscience and Neuropharmacology Research

Abstract Brain pathological features during manic/hypomanic and depressive episodes in the same patients with bipolar disorder (BPD) have not been described precisely. The study aimed to investigate depressive and manic-phase-specific brain neural activity patterns of BPD in the same murine model to provide information guiding investigation of the mechanism of phase switching and tailored prevention and treatment for patients with BPD. In vivo two-photon imaging was used to observe brain activity alterations in the depressive and manic phases in the same murine model of BPD. Two-photon imaging showed significantly reduced Ca 2+ activity in temporal cortex pyramidal neurons in the depression phase in mice exposed to chronic unpredictable mild stress (CUMS), but not in the manic phase in mice exposed to CUMS and ketamine. Total integrated calcium values correlated significantly with immobility times. Brain Ca 2+ hypoactivity was observed in the depression and manic phases in the same mice exposed to CUMS and ketamine relative to naïve controls. The novel object recognition preference ratio correlated negatively with the immobility time in the depression phase and the total distance traveled in the manic phase. With recognition of its limitations, this study revealed brain neural activity impairment indicating that intrinsic emotional network disturbance is a mechanism of BPD and that brain neural activity is associated with cognitive impairment in the depressive and manic phase...