Anti-Inflammatory Effects and Mechanisms of Pudilan Antiphlogistic Oral Liquid

作者：Gang Tian, Xiaoqun Gu, Kaifan Bao, Xuerui Yu, Yuheng Zhang, Yifan Xu, Jie Zheng, Min Hong · 发表于：ACS Omega · 年份：2021 · DOI：10.1021/acsomega.1c04797 · 被引用次数：22 · 研究领域：Inflammasome and immune disorders、Flavonoids in Medical Research、Immune cells in cancer

High Resolution Image Download MS PowerPoint Slide Pudilan antiphlogistic oral liquid (PDL) is a commercial traditional Chinese medicine widely used in the treatment of a variety of inflammatory diseases. However, the specific mechanisms of PDL’s anti-inflammatory effects have not been fully understood. In this research, five classic inflammatory models and a network pharmacology-based strategy were utilized to evaluate its anti-inflammatory efficacy and elucidate its multicomponent and multitarget mode of the anti-inflammatory mechanism. A systems pharmacology approach was carried out via a holistic process of active compound screening, target acquisition, network construction, and further analysis. The potential component–target-associated anti-inflammatory mechanisms of PDL were further verified both in vivo and in vitro . The results showed that PDL exhibited a proven anti-inflammatory effect on multiple types of inflammatory models, including β-hemolytic streptococcus-induced acute pharyngitis, LPS-induced acute lung injury, xylene-induced ear swelling, carrageenan-induced paw edema, and acetic acid-induced capillary permeability-increasing models. Systems pharmacology analysis predicted 45 ingredients of PDL that interact with 185 targets, of which 38 overlapped with the inflammation-related targets. Furthermore, KEGG pathway analysis showed that the predicted targets were mainly involved in hypoxia-inducible factor (HIF)-1, tumor necrosis factor (TNF), nuclear factor k...