Circular RNA circ_0062019 exerts oncogenic properties in prostate cancer via mediating miR‐1253/NRBP1 axis

作者：Xiaojun Ren, Ji-yan Cheng, Min Zhu, Xiao Chen, Min Jiang, Xinmei Hu, Yanan Lu · 发表于：Andrologia · 年份：2021 · DOI：10.1111/and.14343 · 被引用次数：8 · 研究领域：Circular RNAs in diseases、MicroRNA in disease regulation、Heat shock proteins research

Circular RNAs (circRNAs) participate in the progression of various cancers. However, the function of circ_0062019 in prostate cancer (PCa) remains unclear. In this study, CCK-8, colony formation, transwell, tube formation and flow cytometry assays were applied to assess cell proliferation, motility, angiogenesis, cell cycle distribution and apoptosis. The binding association between miR-1253 and circ_0062019 or NRBP1 was verified through dual-luciferase reporter assay and RIP assay. Xenograft assay was conducted to evaluate tumour formation in vivo. As a result, circ_0062019 and NRBP1 were increased, but miR-1253 was decreased in PCa. Depletion of circ_0062019 curbed cell proliferation, migration, invasion, angiogenesis and EMT and induced apoptosis in PCa cells. Circ_0062019 facilitated the malignancy of PCa cells via sequestering miR-1253. Simultaneously, miR-1253 hindered PCa cell progression via regulating NRBP1. Ccirc_0062019 silencing suppressed tumour growth in vivo. Taken together, circ_0062019 expedited PCa progression through mediating miR-1253/NRBP1 pathway.