African Swine Fever Virus Regulates Host Energy and Amino Acid Metabolism To Promote Viral Replication

作者：Xue Qiao, Huisheng Liu, Zixiang Zhu, Fan Yang, Yingying Song, Zongqiang Li, Zhaoning Xue, Weijun Cao, Xiangtao Liu, Haixue Zheng · 发表于：Journal of Virology · 年份：2021 · DOI：10.1128/jvi.01919-21 · 被引用次数：91 · 研究领域：Animal Disease Management and Epidemiology、Viral gastroenteritis research and epidemiology、Viral Infectious Diseases and Gene Expression in Insects

In order to promote viral replication, viruses often cause severe immunosuppression and seize organelles to synthesize a large number of metabolites required for self-replication. African swine fever virus (ASFV) has developed many strategies to evade host innate immune responses. However, the impact of ASFV infection on host cellular metabolism remains unknown. Here, for the first time, we analyzed the metabolomic profiles of ASFV-infected PAMs. ASFV infection increased host TCA cycle and amino acid metabolism. Aspartate, glutamate, and TCA cycle promoted ASFV replication. ASFV infection also induced the increase of lactate production to inhibit innate immune responses for self-replication. This study identified novel immune evasion mechanisms utilized by ASFV and provided insights into ASFV-host interactions, which is critical for guiding the design of new prevention strategies against ASFV targeting the altered metabolic pathways.