Single‐cell transcriptomics of LepR‐positive skeletal cells reveals heterogeneous stress‐dependent stem and progenitor pools

作者：Chunyang Mo, Jingxin Guo, Jiachen Qin, Xiaoying Zhang, Yuxi Sun, Hanjing Wei, Dandan Cao, Yiying Zhang, Chengchen Zhao, Yanhong Xiong, Yong Zhang, Yao Sun, Li Shen, Rui Yue · 发表于：The EMBO Journal · 年份：2021 · DOI：10.15252/embj.2021108415 · 被引用次数：91 · 研究领域：Single-cell and spatial transcriptomics、Immune cells in cancer、Extracellular vesicles in disease

Leptin receptor (LepR)‐positive cells are key components of the bone marrow hematopoietic microenvironment, and highly enrich skeletal stem and progenitor cells that maintain homeostasis of the adult skeleton. However, the heterogeneity and lineage hierarchy within this population has been elusive. Using genetic lineage tracing and single‐cell RNA sequencing, we found that Lepr‐Cre labels most bone marrow stromal cells and osteogenic lineage cells in adult long bones. Integrated analysis of Lepr‐Cre‐traced cells under homeostatic and stress conditions revealed dynamic changes of the adipogenic, osteogenic, and periosteal lineages. Importantly, we discovered a Notch3+ bone marrow sub‐population that is slow‐cycling and closely associated with the vasculatures, as well as key transcriptional networks promoting osteo‐chondrogenic differentiation. We also identified a Sca‐1+ periosteal sub‐population with high clonogenic activity but limited osteo‐chondrogenic potential. Together, we mapped the transcriptomic landscape of adult LepR+ stem and progenitor cells and uncovered cellular and molecular mechanisms underlying their maintenance and lineage specification.