Analysis of the catalase-hydrogen peroxide intermediate in coupled oxidations

作者：Britton Chance, Nozomu Oshino · 发表于：Biochemical Journal · 年份：1973 · DOI：10.1042/bj1310564 · 被引用次数：31 · 研究领域：Enzyme-mediated dye degradation、Electrochemical Analysis and Applications、Metal-Catalyzed Oxygenation Mechanisms

In previous communications, we considered the kinetics of the catalase-H202 intermediate in coupled oxidations ofthe isolated liver catalase (Chance, 1949) and of the mixed mitochondrial-peroxisomal frac- tion (Chance & Oshino, 1971).In those cases, it was possible to record the kinetics of formation of the intermediate by H202 pulses, and solve for the H202 concentration.In most ofthe cases, however, only the analysis of steady-state effects are feasible and will be useful for the further study on the catalase reaction, especially in a system such as the perfused liver.It seems appropriate, therefore, to extend the analysis.The chemical equations for the catalase reactions are: Cat + H202 Cat-H202 (e-p) (x) k.2 (P) Cat-H202 + C2H5OH - Cat + H20 + CH3CHO