The use of 'CO2 buffers' in manometric measurements of cell metabolism

作者：Hans Adolf Krebs · 发表于：Biochemical Journal · 年份：1951 · 被引用次数：61 · 研究领域：Pharmacogenetics and Drug Metabolism、Metabolomics and Mass Spectrometry Studies、Diet and metabolism studies

NECROGENIC YEAST DIETS 349 that necrotic livers from tocopherol-depleted rats fed baker's yeast diet had an even lower glutathione content of about 25 mg./100 g. liver.In conclusion, the nutritive properties of baker's and brewer's yeasts differ in respect to both growthpromoting and necrogenic properties.The aminoacid contents of the two yeasts could explain the difference in growth-promoting properties, the diet containing baker's yeast being the more deficient in several amino-acids.We cannot, however, say whether the small differences in the sulphur-containing amino-acid content account entirely for their different necrogenic properties.Further experiments are needed to establish this point.SUMMARY 1.A comparison was made between the dietetic properties of British baker's yeast and American brewer's yeast, both of which were used as the sole source of protein in the production of dietetic liver necrosis in rats.Diets containing 7-8 % of yeast protein were used.2. When fed to tocopherol-depleted weanling rats the baker's yeast diet proved to be more necrogenic than the brewer's yeast diet.Growth experiments on normal weanlings also showed the inferior biological value of baker's yeast.3. Rats fed the yeast diets for 33 days had liver glutathione contents which were 50 % lower than those ofnormal rats.There was no difference between the composition of livers of rats fed either type of yeast.4. Different dietetic deficiencies exhibited by the two yeasts are discussed in rela...