Structure of the translating Neurospora ribosome arrested by cycloheximide

作者：Lunda Shen, Zhaoming Su, Kailu Yang, Cheng Wu, Thomas Becker, Deborah Bell‐Pedersen, Junjie Zhang, Matthew S. Sachs · 发表于：Proceedings of the National Academy of Sciences · 年份：2021 · DOI：10.1073/pnas.2111862118 · 被引用次数：41 · 研究领域：RNA and protein synthesis mechanisms、Toxin Mechanisms and Immunotoxins、Bacterial Genetics and Biotechnology

Significance The ribosome has the central role in the translation of RNA. The elongation function of most eukaryotic ribosomes is arrested by the drug cycloheximide (CHX). The site of CHX binding is known. In this work, we determine the structure of a translating ribosome of a filamentous fungus arrested by CHX. By doing so, the specific state of the ribosome arrested in translation is revealed. This work is significant because it provides a structural basis to establish CHX’s mechanism of action. It provides a model for a fungal ribosome and insights into the structure and function of eukaryotic ribosomes through comparative analyses of its features with those from other species.