Physiological and transcriptomic insights into adaptive responses of Seriphidium transiliense seedlings to drought stress

作者：Xiqiang Liu, Aiping Chen, Yuxiang Wang, Guili Jin, Yanhui Zhang, Lili Gu, Chenjian Li, Xinqing Shao, Kun Wang · 发表于：Environmental and Experimental Botany · 年份：2021 · DOI：10.1016/j.envexpbot.2021.104736 · 被引用次数：30 · 研究领域：Plant Stress Responses and Tolerance、Plant Molecular Biology Research、Plant nutrient uptake and metabolism

Drought is the most challenging environmental stress factor for grassland ecosystems as the climate change. Seriphidium transiliense, a typical plant in the desert steppe, is widely distributed in northern Xinjiang Region of China. In this study, we analyzed the phenotypic traits, physiological responses, and transcriptional changes in the leaves and roots of S. transiliense, exposed to different levels of drought stress. The morphology of the aboveground and underground portions of S. transiliense changed significantly under drought stress. Additionally, the antioxidants activity and content in leaves and roots increased dramatically. Based on the weighted gene co-expression network analysis (WGCNA) method, we performed a correlation analysis between physiological traits and differentially expressed genes (DEGs) to characterize the key genes and regulatory pathways involved in drought resistance in S. transiliense. A total of 135 and 120 DEGs associated with drought resistance were identified in leaves and roots, respectively. These genes included 38 responsive transcription factors (TFs) belonging to WRKY, AP2/ERF, C2H2, bHLH, MYB, bZIP, NAC, LEA, MADS, and GRAS families that play critical roles in plant responses to abiotic stresses. Furthermore, many receptor protein kinase encoding genes including CBL, CIPK, LRR-RLK, CRK, PI3K, CML, and PP2C involved in the stress signaling responses such as ABA signaling pathway, MAPK signaling pathway, and Ca2+ signal transduction, wer...