RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells

作者：Peiguo Shi, Michael R. Murphy, Alexis O. Aparicio, Jordan S. Kesner, Zhou Fang, Ziheng Chen, Aditi Trehan, Xuebing Wu · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2021 · DOI：10.1101/2021.11.30.470032 · 被引用次数：24 · 研究领域：CRISPR and Genetic Engineering、RNA and protein synthesis mechanisms、RNA regulation and disease

ABSTRACT While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack of tools that are able to target specific cells based on these markers. Here we show that targeting a single marker RNA with CRISPR/RfxCas13d led to collateral transcriptome destruction in human cells, which can be harnessed to inhibit cell proliferation or to suppress cell state transition.