A Multivariate Diagnostic Model Based on Urinary EpCAM-CD9-Positive Extracellular Vesicles for Prostate Cancer Diagnosis

作者：Yibei Dai, Yiyun Wang, Ying Cao, Yu Pan, Lingyu Zhang, Zhenping Liu, Ping Ying, Danhua Wang, Gong Zhang, Yiwen Sang, Xuchu Wang, Zhihua Tao · 发表于：Frontiers in Oncology · 年份：2021 · DOI：10.3389/fonc.2021.777684 · 被引用次数：21 · 研究领域：Extracellular vesicles in disease

Introduction Prostate cancer (PCa) is one of the most frequently diagnosed cancers and the leading cause of cancer death in males worldwide. Although prostate-specific antigen (PSA) screening has considerably improved the detection of PCa, it has also led to a dramatic increase in overdiagnosing indolent disease due to its low specificity. This study aimed to develop and validate a multivariate diagnostic model based on the urinary epithelial cell adhesion molecule (EpCAM)-CD9–positive extracellular vesicles (EVs) (uEV EpCAM-CD9 ) to improve the diagnosis of PCa. Methods We investigated the performance of uEV EpCAM-CD9 from urine samples of 193 participants (112 PCa patients, 55 benign prostatic hyperplasia patients, and 26 healthy donors) to diagnose PCa using our laboratory-developed chemiluminescent immunoassay. We applied machine learning to training sets and subsequently evaluated the multivariate diagnostic model based on uEV EpCAM-CD9 in validation sets. Results Results showed that uEV EpCAM-CD9 was able to distinguish PCa from controls, and a significant decrease of uEV EpCAM-CD9 was observed after prostatectomy. We further used a training set (N = 116) and constructed an exclusive multivariate diagnostic model based on uEV EpCAM-CD9 , PSA, and other clinical parameters, which showed an enhanced diagnostic sensitivity and specificity and performed excellently to diagnose PCa [area under the curve (AUC) = 0.952, P < 0.0001]. When applied to a validation test (N ...