Dietary Amylose/Amylopectin Ratio Modulates Cecal Microbiota and Metabolites in Weaned Goats

作者：Kefyalew Gebeyew, Kai Chen, Teketay Wassie, Md. Abul Kalam Azad, Jianhua He, Weimin Jiang, Song Wu, Zhixiong He, Zhiliang Tan · 发表于：Frontiers in Nutrition · 年份：2021 · DOI：10.3389/fnut.2021.774766 · 被引用次数：18 · 研究领域：Gut microbiota and health、Ruminant Nutrition and Digestive Physiology、Milk Quality and Mastitis in Dairy Cows

Increasing the ratio of amylose in the diet can increase the quantity of starch that flows to the large intestine for microbial fermentation. This leads to the alteration of microbiota and metabolite of the hindgut, where the underlying mechanism is not clearly understood. The present study used a combination of 16S amplicon sequencing technology and metabolomics technique to reveal the effects of increasing ratios of amylose/amylopectin on cecal mucosa- and digesta-associated microbiota and their metabolites in young goats. Twenty-seven Xiangdong black female goats with average body weights (9.00 ± 1.12 kg) were used in this study. The goats were randomly allocated to one of the three diets containing starch with 0% amylose corn (T1), 50% high amylose corn (T2), and 100% high amylose corn (T3) for 35 days. Results showed that cecal valerate concentration was higher ( P < 0.05) in the T2 group than those in the T1 and T3 groups. The levels of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 were decreased ( P < 0.05) in cecal tissue while IL-10 was increased ( P < 0.05) in the T2 group when compared with T1 or T3 groups. At the phylum level, the proportion of mucosa-associated Spirochaetes was increased ( P < 0.05), while Proteobacteria was deceased by feeding high amylose ratios ( P < 0.05). The abundance of Verrucomicrobia was decreased ( P < 0.05) in the T3 group compared with the T1 and T2 groups. The abundance of digesta-as...