In vivo maternal haploid induction in tomato

作者：Yu Zhong, Baojian Chen, Dong Wang, Xijian Zhu, Mengran Li, Jinzhe Zhang, Ming Chen, Min Wang, Tjitske Riksen, Li Li, Xiaolong Qi, Yuwen Wang, Dehe Cheng, Zongkai Liu, Jinlong Li, Chen Chen, Yanyan Jiao, Wenxin Liu, Sanwen Huang, Chenxu Liu, Kim Boutilier, Shaojiang Chen · 发表于：Plant Biotechnology Journal · 年份：2021 · DOI：10.1111/pbi.13755 · 被引用次数：92 · 研究领域：Plant tissue culture and regeneration、Chromosomal and Genetic Variations、Plant Virus Research Studies

Tomato (Solanum lycopersicon) is the second largest vegetable crop and the largest fruit crop (Costa and Heuvelink, 2018). Progress in tomato breeding has been achieved by classical breeding, introgression of traits found in related wild Solanum species and exploiting heterosis in F1 hybrid crosses (Lin et al., 2014). These approaches require the development of inbred lines to reduce or largely eliminate heterozygosity. Classically, multiple rounds of selfing or backcrossing are used to generate inbred lines (Gale, 1980), but homozygous lines can also be obtained in a single generation using doubled haploid (DH) technology (Jacquier et al., 2020). However, tomato is highly recalcitrant for haploid induction (HI) (Seguí-Simarro, 2010). Significant breakthroughs in DH production were made after identification of the zmpla1/mtl/nld and zmdmp mutant genes that induce in vivo maternal haploid embryos in maize and extension of this technology to other monocot crops (Jacquier et al., 2020 and references therein). The utility of dmp mutants for HI in dicots was demonstrated in Arabidopsis (Zhong et al., 2020), but it is not known whether this approach can be applied in dicot crops. Here we show that dmp mutants can also be used for efficient and genotype-independent maternal HI in tomato. The tomato genome contains a single DMP gene (SlDMP; Solyc05g007920) that is highly expressed in pollen and flower buds (Zhong et al., 2020). We generated sldmp mutants in cv. Ailsa Craig using a CR...