The quantitative proteome atlas of a model cyanobacterium

作者：Jinlong Wang, Xiahe Huang, Haitao Ge, Yan Wang, Yan Wang, Weiyang Chen, Li‐Min Zheng, Chengcheng Huang, Haomeng Yang, Lingyu Li, Na Sui, Yu Wang, Yu Wang, Yuanya Zhang, Dandan Lu, Longfa Fang, Wu Xu, Yuqiang Jiang, Fang Huang, Yingchun Wang, Yingchun Wang · 发表于：Journal of genetics and genomics/Journal of Genetics and Genomics · 年份：2021 · DOI：10.1016/j.jgg.2021.09.007 · 被引用次数：24 · 研究领域：Photosynthetic Processes and Mechanisms、Metabolomics and Mass Spectrometry Studies、Genomics and Phylogenetic Studies

Cyanobacteria are a group of oxygenic photosynthetic bacteria with great potentials in biotechnological applications and advantages as models for photosynthesis research. The subcellular localizations of the majority of proteins in any cyanobacteria remain undetermined, representing a major challenge in using cyanobacteria for both basic and industrial researches. Here, using label-free quantitative proteomics, we map 2027 proteins of Synechocystis sp. PCC6803, a model cyanobacterium, to different subcellular compartments and generate a proteome atlas with such information. The atlas leads to numerous unexpected but important findings, including the predominant localization of the histidine kinases Hik33 and Hik27 on the thylakoid but not the plasma membrane. Such information completely changes the concept regarding how the two kinases are activated. Together, the atlas provides subcellular localization information for nearly 60% proteome of a model cyanobacterium, and will serve as an important resource for the cyanobacterial research community.