From bulk, single-cell to spatial RNA sequencing

作者：Xinmin Li, Cun‐Yu Wang · 发表于：International Journal of Oral Science · 年份：2021 · DOI：10.1038/s41368-021-00146-0 · 被引用次数：635 · 研究领域：Single-cell and spatial transcriptomics、Cancer Genomics and Diagnostics、Extracellular vesicles in disease

RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA sequencing (RNAseq), popular single cell RNA sequencing (scRNAseq) to newly emerged spatial RNA sequencing (spRNAseq). Bulk RNAseq studies average global gene expression, scRNAseq investigates single cell RNA biology up to 20,000 individual cells simultaneously, while spRNAseq has ability to dissect RNA activities spatially, representing next generation of RNA sequencing. This article highlights these technologies, characteristic features and suitable applications in precision oncology.