Effects of Plateau Air Quality and Low Oxygen Content on Platelet mtDNA Methylation and High Altitude Pulmonary Edema

作者：Ziquan Liu, Huanhuan Cui, Lei Zhao, Fucai Ma, Yanqing Liu, Yuansen Chen, Jiale Chen, Yaning Jia, Wenli Li, Jinxia Cai, Bi Xun, Penghui Li, Haojun Fan, Liqiong Guo, Shike Hou · 发表于：Aerosol and Air Quality Research · 年份：2021 · DOI：10.4209/aaqr.210222 · 被引用次数：4 · 研究领域：High Altitude and Hypoxia、Cardiovascular and Diving-Related Complications、Neuroscience of respiration and sleep

In 2020, the monitored AQI (Air Quality Index) in plateau – Tibet varied from 24 to 98 and averaged 48, while the PM2.5 concentration ranged between 3.0 and 31 μg m-3 and with an average of 10 μg m-3. The plateau - Tibet, had a good air quality, but with a lower atmospheric pressure and low oxygen concentration. As the important characteristic of plateau atmospheric environment, hypobaric hypoxia (HBH) is the key risk factors causing high altitude pulmonary edema (HAPE). It has been reported that the abnormal platelet function caused by HBH is an important pathogenesis of HAPE. In this preliminary study, male Sprague-Dawley rats were randomly divided into control group and HBH 24, 48, and 72-hour exposed groups. A multi-functional hypoxic chamber was used to simulate HAPE model with an altitude of 6000 m, oxygen concentration of 9.46% and partial oxygen pressure of 9.6 KPa. We measured pulmonary wet to dry (W/D) weight ratio, MT-Cox1, MT-Cox2 and MT-Cox3 methylation levels by pyrosequencing on blood samples from all rats and the levels of plasma inflammatory mediators by Luminex liquid chip technology. Results showed that the W/D ratio increased in the HBH-48h and HBH-72h exposed groups significantly (p < 0.05). The mtDNA methylation levels in all HBH exposed groups were lower than pre-exposure (all p value < 0.05). The levels of MT-Cox1, MT-Cox2_Pos2 and MT-Cox2_Pos3 methylation were correlated with W/D ratio significantly (r = 0.16-0.20, p < 0.05). And a strong correlation ...